abstract：:We have used the complex of antigen with class II major histocompatibility proteins (Ia) in membrane-bound form to target a phototoxic compound to antigen-specific T cell hybridomas in vitro. The iodoacetamidyl ester of phototoxic pyrene was bound covalently to antigen-presenting cells (APC), and protein antigens were...

journal_title：Photochemistry and photobiology

authors： Chang NH,Rayner DC,Boggs JM

更新日期：1995-05-01 00:00:00

abstract：:Collagen was exposed to an ultraviolet (UV) lamp that emitted predominantly in the UVB range. The cross-linking of collagen type I and type IV by UV irradiation was observed. Amino acid analyses revealed that Tyr residues in both collagen types I and IV were decreased by irradiation. In collagen type IV, losses of His...

journal_title：Photochemistry and photobiology

authors： Kato Y,Nishikawa T,Kawakishi S

更新日期：1995-04-01 00:00:00

abstract：:Activation of expression of the heme oxygenase (HO) gene appears to be involved in a cellular defense system in mammalian cells. We now demonstrate that while HO-1 mRNA levels are strongly inducible in dermal fibroblasts they are barely inducible in human epidermal keratinocytes following oxidative stress (UVA radiati...

journal_title：Photochemistry and photobiology

authors： Applegate LA,Noël A,Vile G,Frenk E,Tyrrell RM

更新日期：1995-03-01 00:00:00

abstract：:The sequence specificity of photobinding to DNA of two tetrahydrobenzopsoralen derivatives has been investigated by testing the photoreactivity toward a number of self-complementary oligonucleotides. The thermodynamic constant for noncovalent binding to each DNA sequence was evaluated. The extent of photoreactivity wa...

journal_title：Photochemistry and photobiology

authors： Mobilio S,Tondelli L,Capobianco M,Gia O

更新日期：1995-02-01 00:00:00

abstract：:Experimental studies have recently demonstrated that fluorescence emission can be quenched by laser light pulses from modern high-repetition rate lasers, a phenomenon we call "light quenching." In this overview article, we describe the possible effects of light quenching on the steady-state and time-resolved intensity...

journal_title：Photochemistry and photobiology

authors： Lakowicz JR,Gryczyński I,Kuśba J,Bogdanov V

更新日期：1994-12-01 00:00:00

abstract：:There is a need to improve the selectivity of photodynamic therapy and for better targeting of tumor cells within specific tumor compartments. Selective in vitro phototoxicity of a human bladder carcinoma cell line 647V has been achieved by targeting sulfonated aluminum phthalocyanines (AlSPc) with monoclonal antibodi...

journal_title：Photochemistry and photobiology

authors： Morgan J,Lottman H,Abbou CC,Chopin DK

更新日期：1994-11-01 00:00:00

abstract：:Antisense oligodeoxynucleotides directed against a 24-mer RNA derived from the long terminal repeat (LTR) region of HIV were linked to proto- and methylpyrroporphyrin and their zinc derivatives. The oligonucleotide-porphyrin conjugates were tested for their ability to induce photodamage on the target RNA. Upon hybridi...

journal_title：Photochemistry and photobiology

authors： Mastruzzo L,Woisard A,Ma DD,Rizzarelli E,Favre A,Le Doan T

更新日期：1994-10-01 00:00:00

abstract：:The immunological consequences of exposure to UVA (320-400 nm) radiation are unclear. This study describes the relationship between the generation of epidermal cis-urocanic acid and the ability to respond to a contact-sensitizing agent, in hairless mice exposed to different UV radiation sources, which incorporate succ...

journal_title：Photochemistry and photobiology

authors： Reeve VE,Boehm-Wilcox C,Bosnic M,Cope R,Ley RD

更新日期：1994-09-01 00:00:00

abstract：:We used GHz frequency-domain fluorometry to investigate the time-dependent intensity decays of N-acetyl-L-trytophanamide (NATA) when collisionally quenched by acrylamide in propylene glycol at 20 degrees C. The intensity decays of NATA became increasingly heterogeneous in the presence of acrylamide. The NATA intensity...

journal_title：Photochemistry and photobiology

authors： Lakowicz JR,Zelent B,Gryczynski I,Kuśba J,Johnson ML

更新日期：1994-09-01 00:00:00

abstract：:The crystal and molecular structure of the title compound 4 have been determined by single crystal X-ray crystallography. The structure shows the typical S4-ruffled conformation observed for Ni(II) tetrapyrroles. Compared to the structure of the methyl ester of (13(2)-demethoxycarbonyl-pheophorbidato a)nickel(II), 4 s...

journal_title：Photochemistry and photobiology

authors： Senge MO,Smith KM

更新日期：1994-08-01 00:00:00

abstract：:The individual tryptophanyl contributions to the near-ultraviolet dichroic activity of apomyoglobin in its native conformation have been resolved. This was accomplished by comparing the spectra of two classes of apomyoglobin with different aromatic residue contents and observing the effect of a specific modification o...

journal_title：Photochemistry and photobiology

authors： Sirangelo I,Irace G,Bismuto E

更新日期：1994-06-01 00:00:00

abstract：:The photobiologic properties of a chlorin-thiobarbiturate conjugate were examined. In addition to the characteristic 670 nm chlorin absorbance, the conjugate exhibited new absorbance bands at 517 and 743 nm, but these were not a part of the fluorescence emission spectra, nor was fluorescence observed at wavelengths > ...

journal_title：Photochemistry and photobiology

authors： Kessel D,Morgan A

更新日期：1994-05-01 00:00:00

abstract：:The mode of cell death following photodynamic therapy was investigated from the perspective of programmed cell death or apoptosis. Human prostate carcinoma cells (PC3), human non-small cell lung carcinoma (H322a) and rat mammary carcinoma (MTF7) were treated by photodynamic therapy. An examination of extracted cellula...

journal_title：Photochemistry and photobiology

authors： He XY,Sikes RA,Thomsen S,Chung LW,Jacques SL

更新日期：1994-04-01 00:00:00

abstract：:A concept has been presented whereby X-ray irradiation can be used to induce visible luminescence of chromophores such as porphyrins. The essential aspects of the idea have been demonstrated in a supramolecular assembly consisting of a water-dispersed polystyrene latex with an embedded scintillating compound, 2,5-diph...

journal_title：Photochemistry and photobiology

authors： Kokotov S,Lewis A,Neumann R,Amrusi S

更新日期：1994-03-01 00:00:00

abstract：:Extracellular pH was measured continuously and noninvasively in hairless mice using a transcutaneous spectrofluorometric technique and a fluorescent pH probe, bis-carboxyethyl carboxyfluorescein (BCECF). The acid form of BCECF was injected intravenously. An optical fiber system excited the fluorescent probe at 450 and...

journal_title：Photochemistry and photobiology

authors： Russell DA,Pottier RH,Valenzeno DP

更新日期：1994-03-01 00:00:00

abstract：:Gel sequencing experiments with the 5'- and 3'-end-labeled oligonucleotides d(A3GA4GA5GA6GA3G) and d(AT)10 have demonstrated that dimeric adenine photoproducts and thymine-adenine photoadducts constitute alkali-labile lesions in UV-irradiated DNA. On treatment with hot piperidine, DNA strand breakage occurs predominan...

journal_title：Photochemistry and photobiology

authors： Hejmadi V,Stevenson C,Kumar S,Davies RJ

更新日期：1994-02-01 00:00:00

abstract：:Exposure of cultured MRC5 human fibroblasts or NCTC 2544 human keratinocytes to mild doses of ultraviolet A (UVA: 320-400 nm) radiations markedly decreased the actin reactivity with fluorescein-labeled phalloidin. This indicates a change in the degree of polymerization of actin and thus in the organization of actin fi...

journal_title：Photochemistry and photobiology

authors： Djavaheri-Mergny M,Pieraggi MT,Mazière C,Santus R,Lageron A,Salvayre R,Dubertret L,Mazière JC

更新日期：1994-01-01 00:00:00

abstract：:Aqueous solutions of KI were examined for use as chemical actinometers to measure 254 nm (germicidal) radiation. Irradiation results in electron ejection from iodide such that aqueous electrons and iodine atoms are formed. In the presence of N2O, an electron scavenger, recombination of these two reactive species is el...

journal_title：Photochemistry and photobiology

authors： Rahn RO

更新日期：1993-12-01 00:00:00

abstract：:The proteins induced by heat and other stressors, called heat shock proteins (HSP) or stress proteins, are considered to play a general role in protection from cellular injury. Exposure to UVA (320-400 nm) following application of 8-methoxypsoralen (8-MOP), termed PUVA is commonly used in the field of dermatology. In ...

journal_title：Photochemistry and photobiology

authors： Muramatsu T,Yamashina Y,Tada H,Kobayashi N,Yamaji M,Ohno H,Shirai T,Takahashi A,Ohnishi T

更新日期：1993-12-01 00:00:00

abstract：:The in vitro cell survival, localization and ultrastructural changes following irradiation were examined in 9L glioma cells sensitized with a new photosensitizer, lysyl chlorin p6 (LCP). In clonogenic assays, LCP was 10-100-fold more phototoxic than photofrin II on a microgram/mL basis. Lysyl chlorin p6 uptake was blo...

journal_title：Photochemistry and photobiology

authors： Leach MW,Higgins RJ,Autry SA,Boggan JE,Lee SJ,Smith KM

更新日期：1993-11-01 00:00:00

abstract：:Ultraviolet A radiation participates in cytotoxicity and carcinogenesis of the skin by a mechanism involving the generation of reactive oxygen species. Endogenous antiradical defense systems utilize metalloenzymes including Se-dependent glutathione peroxidase and Cu and Zn superoxide dismutase. The aim of the present ...

journal_title：Photochemistry and photobiology

authors： Leccia MT,Richard MJ,Beani JC,Faure H,Monjo AM,Cadet J,Amblard P,Favier A

更新日期：1993-10-01 00:00:00

abstract：:The phototoxic anti-hyperlipoproteinemic drug fenofibrate was found to be photolabile under aerobic and anaerobic conditions. Irradiation under argon of a methanol solution of this drug produced the photoproducts isopropyl 4-(1-[4-chlorophenyl]-1,2-dihydroxy)ethylphenoxyisobutyrate, 1,2-bis(4-chlorophenyl)-1,2bis (4-[...

journal_title：Photochemistry and photobiology

authors： Vargas F,Canudas N,Miranda MA,Boscá F

更新日期：1993-10-01 00:00:00

abstract：:Tetracycline molecules offer several sites for peroxidative metabolism of the type known to lead to oxygen consumption and electronic excitation. Accordingly, when tetracycline and chlortetracycline were exposed to horseradish peroxidase in the presence of hydrogen peroxide, oxygen was taken up and light emission was ...

journal_title：Photochemistry and photobiology

authors： Gilio VE,Cilento G

更新日期：1993-09-01 00:00:00

abstract：:Liposomes were prepared from mixtures of dipalmitoyl-L-alpha-phosphatidylcholine and up to 40% mol:mol of N-stearoyl-L-histidine (NSH) in the presence of the hydrophobic sensitizer DHE. In the dark such liposomes are stable and retain entrapped salts. On photolysis with visible light, liposomes leak trapped ions at NS...

journal_title：Photochemistry and photobiology

authors： Chowdhary RK,Green CA,Morgan CG

更新日期：1993-09-01 00:00:00

abstract：:We have previously demonstrated the ability of UVC (254 nm) radiation to induce asynchronous polyoma replication in rat fibroblast cells (H3 line) that contain an integrated copy of polyoma virus. In the present study we show that general purpose lamps can induce polyoma replication in these cells as well. The amount ...

journal_title：Photochemistry and photobiology

authors： Rutberg SE,Ronai Z

更新日期：1993-08-01 00:00:00

abstract：:Biophysical and photobiological properties of a group of bacteriochlorins were compared with efficacy of these products for photodynamic therapy of murine tumors. Predictive factors for selective photosensitization in vivo include affinity binding to lipoproteins greater than albumin, extinction coefficient at the wav...

journal_title：Photochemistry and photobiology

authors： Kessel D,Smith KM,Pandey RK,Shiau FY,Henderson B

更新日期：1993-08-01 00:00:00

abstract：:The capacity of some linear and angular furocoumarins to induce DNA-protein cross-links by UVA (320-400 nm) irradiation has been evaluated in Chinese hamster ovary cells. Two linear furocoumarins, psoralen and 8-methoxypsoralen appeared to be capable of inducing DNA-protein cross-links to a noticeable extent. 4'-Methy...

journal_title：Photochemistry and photobiology

authors： Bordin F,Carlassare F,Busulini L,Baccichetti F

更新日期：1993-07-01 00:00:00

abstract：:Copper(II), in the presence of UV-B radiation (280-315 nm), can generate single-strand breaks in the sugar-phosphate backbone of pBR322 plasmid DNA. A low level of single-strand backbone breaks occurs in the presence of Cu(II) alone, but UV-B irradiation increases the rate by the more than 100-fold. Concomitant with t...

journal_title：Photochemistry and photobiology

authors： Lloyd RE,Larson RA,Adair TL,Tuveson RW

更新日期：1993-06-01 00:00:00

abstract：:We have compared the cytotoxicity of daunomycin in vitro to highly differentiated normal epithelial cells (Fisher rat thyroid cells, FRTL-5) and to two neoplastic cell lines, a thyroid carcinoma (TK-6) and its lung metastasis (MPTK-6). Whereas the cell lines are equally sensitive to the drug in the dark, if irradiated...

journal_title：Photochemistry and photobiology

authors： Andreoni A,Colasanti A,Kisslinger A,Mastrocinque M,Portella G,Riccio P,Roberti G

更新日期：1993-05-01 00:00:00

abstract：:Intracellular targets for the photosensitizer alpha-terthienyl (alpha T) were examined by fluorescence microscopy and microfluorospectrometry using human nonkeratinized buccal cells. Intracellular distribution of alpha T was observed as fluorescent patches widely dispersed in the cytoplasm. The distribution of the flu...

journal_title：Photochemistry and photobiology

authors： Sasaki M,Koyama S,Tokiwa K,Fujita H

更新日期：1993-05-01 00:00:00

abstract：:Molecular cloning techniques were employed to obtain hybrid luciferases with their alpha and beta subunits encoded by luxA and luxB genes, respectively, from Xenorhabdus luminescens strain HW or Vibrio fischeri. Although the two wild-type luminous bacteria are phylogenetically diverged, the hybrid luciferase Xf compri...

journal_title：Photochemistry and photobiology

authors： Xi L,Tu SC

更新日期：1993-04-01 00:00:00

abstract：:A new water-soluble porphyrin derivative, 2,4-bis(1-decyloxyethyl)-deuteroporphyrinyl-6,7-bisaspart ic acid (C10-DP), and its metal complexes (Ga, I(n), Zn, Mn, Cu, Ni and Fe) were examined for their physicochemical properties (absorption, fluorescence, triplet lifetime and partition coefficient) and photocytotoxicity...

journal_title：Photochemistry and photobiology

authors： Ando T,Irie K,Koshimizu K,Takemura T,Nishino H,Iwashima A,Takeda N,Nakajima S,Sakata I

更新日期：1993-04-01 00:00:00

abstract：:Singlet oxygen reacts preferentially with three amino acids in proteins, His, Trp and Met. In order to study the specific molecular events that result from such oxidations, calf alpha-crystallin was photooxidized in the presence of uroporphyrin and the reactions were investigated by high performance liquid chromatogra...

journal_title：Photochemistry and photobiology

authors： Dillon J,Chiesa R,Wang RH,McDermott M

更新日期：1993-03-01 00:00:00

abstract：:Three substituted tetraazaporphyrins, octa-(4-tert-butylphenyl)-tetrapyrazinoporphyrazine, tetra-(4-tert-butyl)phthalocyanine and tetra-(4-tert-butyl)phthalocyanatomagnesium(t4-PcMg), were spectroscopically checked in solutions and liposomes with respect to suitability as potential sensitizers of a possible new start ...

journal_title：Photochemistry and photobiology

authors： Teuchner K,Pfarrherr A,Stiel H,Freyer W,Leupold D

更新日期：1993-03-01 00:00:00

abstract：:A 24 h preculture of HT29-18 human colonic adenocarcinoma cells with the sterol synthesis inhibitor lovastatin at concentrations of 0.1-0.5 microM markedly increased the photocytotoxic effect of photofrin II delivered to cells by low density lipoproteins. Under the same conditions, LDL binding and photofrin II (PII) u...

journal_title：Photochemistry and photobiology

authors： Biade S,Mazière JC,Mora L,Santus R,Mazière C,Auclair M,Morlière P,Dubertret L

更新日期：1993-02-01 00:00:00

abstract：:Previous studies have shown that natural killer (NK) cell activity was suppressed in volunteer subjects exposed to ultraviolet radiation (UVR) from solarium lamps. The present studies were carried out to determine the spectrum of UVR responsible for suppression of NK activity and to develop in vitro methods to analyze...

journal_title：Photochemistry and photobiology

authors： Hersey P,Magrath H,Wilkinson F

更新日期：1993-02-01 00:00:00

abstract：:Types of photoreceptor cells in the retinas of 36 species of vertebrates (5 classes, 14 orders) were investigated immunocytochemically with monoclonal antibodies against chicken iodopsin (Io-mAb) and antiserum against bovine rhodopsin (Rh-As). In mammals, Rh-As labeled the outer segments of some photoreceptor cells in...

journal_title：Photochemistry and photobiology

authors： Kawata A,Oishi T,Fukada Y,Shichida Y,Yoshizawa T

更新日期：1992-12-01 00:00:00

abstract：:A novel antibody-fluorescence method has been developed to elucidate the chromophore topography in phytochrome as it undergoes a photochromic transformation. Förster energy transfer from N-terminal bound, fluorescently labeled Oat-25 Fab antibody fragments to the phytochrome chromophore was measured. The results sugge...

journal_title：Photochemistry and photobiology

authors： Farrens DL,Cordonnier MM,Pratt LH,Song PS

更新日期：1992-11-01 00:00:00

abstract：:Quantitative and qualitative changes in dermal collagen and elastin occur in response to chronic ultraviolet (UV) irradiation. These changes have been implicated in the genesis of the wrinkling seen in chronically irradiated, or photoaged skin. We examined the relationship between wrinkle formation and changes in derm...

journal_title：Photochemistry and photobiology

authors： Moloney SJ,Edmonds SH,Giddens LD,Learn DB

更新日期：1992-10-01 00:00:00

abstract：:We have studied the damage of alcohol dehydrogenase (ADH) and glyceraldehyde 3-phosphate dehydrogenase (GAPD) induced by Fe++/EDTA + H2O2 in combination with UV-A (main output at 365 nm). Enzyme inactivation, formation of hydroxyl radicals (measured in the absence of enzymes), increase in protein carbonyls, oxidation ...

journal_title：Photochemistry and photobiology

authors： Hu ML,Tappel AL

更新日期：1992-09-01 00:00:00